Sentence examples for vector supplied with from inspiring English sources
The linearised vector supplied with the kit has a single, overhanging 3′deoxythymidine (T) residue, allowing efficient ligation between PCR product and vector.
Only the final cloning step was modified so that instead of using the λ TriplEx2 vector supplied with the kit, the size fractionated cDNA was ligated into pGEM-T Easy (Promega, Madison, WI, USA) as per manufacturer's instructions, and transformed into XL10 Gold ultracompetent cells (Stratagene, La Jolla, CA, USA) according to the manufacturer's protocol.
PCR products were gel purified using the BioSpin Gel Extraction Kit (Bioer Technology Co., Ltd) and cloned into pJET1.2 vector supplied with the CloneJET PCR cloning kit (Fermentas) and sequenced from the two ends using BigDye reagenton an ABI3700 sequencer (Applied Biosystems).
Only the final cloning step was modified so that instead of using the λ TriplEx2 vector supplied with the kit, the size fractionated cDNA was ligated into pGEM-T Easy (Promega, Madison, WI, USA) according to the manufacturer's instructions, and transformed into XL10 Gold ultracompetent cells (Stratagene, La Jolla, CA, USA) according to the manufacturer's protocol.
PCR products were gel purified using the BioSpin Gel Extraction Kit (Bioer Technology Co., Ltd) and cloned into pJET1.2 vector supplied with the CloneJET PCR cloning kit (Fermentas) and sequenced from the two ends using BigDye reagent in ABI3700 sequencer (Applied BioSystems).
Lentiviral plasmids were packaged in 293FT cells (Invitrogen) using vectors supplied with the ViraPower Expression kit (Invitrogen, San Diego, CA).
The 0.5-8 kb fraction was ligated into an SfiI digested, dephosphorylated pDNR-LIB vector as supplied with the Creator™ SMART™ cDNA library construction kit.
The membranes were incubated overnight at 4°C with c-Myc antibody (1∶1,000, Clontech, supplied with c-Myc vector) and then re-probed with GAPDH antibody (1∶300, Chemicon, Temecula, CA) to assess the protein amount.
The supersensitive detection system (BioGenex, Munich, Germany) was used and the immunoreaction was developed in the diamino-benzidine (DAB) supplied with the kit (Vector lab, Burlingame, CA).
Sequencing reactions were performed using the Big Dye kit (Applied Biosystems) and the M13 F primer supplied with the pCR4 TOPO vector (Invitrogen).
Briefly, COS1 cells transfected with the p3.1M- DLK1-HA vector were harvested in the Phosphate Buffered Saline (GIBCO) supplied with 1% Igepal CA-630 and the complete protease inhibitor cocktail.
