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Our vector suite approach shows that rapid expression survey can be achieved within the baculovirus system and in addition, goes some way to identifying the underlying basis of the expression level obtained.
These entry clones are part of the current vector suite release (See table page 24 of Manual S1).
The aim of this work was to generate a versatile vector suite allowing the mid- to high-throughput analysis of cis-regulatory sequences and protein function.
In addition, the Chordate vector suite allows to add either an N- or C-terminal Venus-YFP or CyanFP fluorescent protein to any gene product and thus to track the sub-cellular localisation of proteins of interest.
The vector suite was designed to facilitate an efficient and parallel cloning workflow of candidates as fusions to partners.
In summary, the vector suite facilitates a low-temperature optimized system for heterologous expression of putatively cold-active enzymes in fusion to both small and large fusion partners.
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The Vector NTI suite of programs was used to confirm the previous analysis.
Multiple sequence alignment was conducted by using the Vector NTI suite 11 (Invitrogen).
Multiple sequence alignments were carried out with Align X using the software program Vector NTITM Suite 9 (InforMax) and the GCG Wisconsin Package (Accelerys).
Multiple sequence alignment was conducted by using the Vector NTI suite 8. Phylogenetic trees were constructed by using the DNAMAN package.
Vector NTI suite 11 (InforMax, Inc).
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