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Recently, reverse genetic and crystallographic studies on GFLV revealed that a positively charged pocket in the CP B domain located at the virus surface may be responsible for vector specificity.
However, the majority of the previously described determinants of vector specificity of different alphaviruses are located within the E2 glycoprotein, circumstantially indicating that the process of alphavirus adaptation to new mosquito species occurs via adaptation to a specific cell surface receptor expressed in this mosquito [27] [31].
Thus, plenty of scope remains for further exploration of vector specificity by subpopulations of the pathogen.
The vector specificity of the technique and its sensitivity to reflect levels of exposure in humans has not been addressed.
Such vector specificity might play a role in augmenting the genetic isolation between the two mcy clades.
Similarly, it is currently not known whether the two groups of CpCSV isolates also differ in aphid vector specificity.
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It remains to be shown whether there are also consistent differences between FBNYV and FBNSV isolates in host range, symptomatology, and aphid vector specificities.
Early investigations questioned host and vector specificities of relapsing fever spirochetes; studies were carried out that enabled ticks to feed on patients with the louse-borne form of the disease and subsequently letting these ticks feed upon nonfebrile persons.
While this interaction has an unclear mechanism, it seems to be implicated in the insect-vector specificity [18].
It will also facilitate studies to elucidate the genetics underlying gene expression in pest- and non-pest biotypes, and the basis for virus-vector specificity, resistance to insecticides, and plant host preferences for this cryptic species.
Finally, the challenges in bringing miRNA antagonists to the clinical arena are similar to the ones we encountered with gene therapy, namely the mode of delivery, vectors, specificity, and toxicity in vivo [ 34].
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com