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Because Babesia species are both host and vector specific, tick control plays an important role in reducing incidence.
Each cDNA was bacterially amplified, isolated, and sequenced with vector specific primers (M13F and M13R).
The clones were initially sequenced with M13 forward and reverse vector specific primers.
Two PCR were performed with each one of the mutagenic primers and the corresponding vector specific primer.
Single PCR fragments gel-purified and assembled in a subsequent PCR using vector specific primers to generate the full-length mutant DNA.
Constructs containing Ac-DAF-16 bound fragments from the in vitro genomic selection cloned in pBluescript KS+/pGEM-T were sequenced with vector specific primer T7.
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Positive clones were screened using gene and vector-specific primers and confirmed by sequencing.
Furthermore, intramuscular immunization did not induce antigen-specific mucosal antibodies while intranasal inoculation stimulated vector-specific IgA antibodies in vaginal washings and serum.
Study design: Until recently, the Chinese ADRV strain was the only HuGBR strain that had been partially sequenced by cloning various viral genes using vector-specific primers.
The obtained fragments were cloned into the pMD18-T vector (Takara, Dalian, China) and prepared for DNA sequencing using vector-specific primers.
Inserts were sequenced with vector-specific T7 primer.
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