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Indeed, only the HAWW_5 protein when non-denatured was able to interact with the vector, showing that it is properly folded, functional and might be a good starting material for influenza vaccine construction.
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Expression of one of the two cDNAs in insect cells with a baculovirus vector shows that this cDNA encodes an active amylase with a mobility that corresponds to that of one of the two isozymes present in larval extracts.
Use of a carboxyl-terminal GFP tagging vector showed that fluorescence measurement paralleled expression level and was a convenient readout in the context of insect cell expression, an observation that was further supported with additional non-kinase targets.
Analysis of several transgenic mice harboring a chimeric Ii cDNA placed in this vector showed that it can drive expression of the reporter protein to levels comparable to those of endogenous Ii. Furthermore, its expression pattern overlaps quite well with that of endogenous Ii.
A high vertex degree for a given vector shows that it could potentially exploit many different mammals.
Stem stability evaluated with the total rotational migration (rotation vector) showed that 17 patients achieved osseointegration at 3 months.
The approach taken for T7, using 1 copy centromeric plasmid vector, showed that 4% of all constructs was able to auto-activate.
Direct comparison of bulk HEK 293 cell cultures generated with a "conventional" vector or with a BAC-based vector showed that the BAC-based vector improved the protein yield by a factor of 10.
Northern blotting analysis of RNA extracted from cells transfected with the U6 promoter-driven sgRNA plasmid expression vector showed that the sgRNA is indeed expressed, and that their stability is enhanced by the presence of Cas9.
Further analysis of stable cell clones harboring the BAC-based vector showed that the protein production was directly proportional to the number of integrated BAC copies and that the protein production was stable for at least 30 passages.
Contrary to what we observed for the self-contained vector, analysis of the autoregulated MOV-scT6cA vector showed that M2-mRNA levels in the off-state were approximately doubled (0.5 vs. 1.2) in the enriched population and strongly increased upon induction at low Dox concentration.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com