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The pbTy vector was used to construct the pbSy vector (see below) and to clone entire ABC transporter genes at PmlI/AscI sites in order to replace bceAB genes.
Interestingly, similar yet weaker effects were found for the self-contained vector (see below).
The remaining insertions were generated with a piggyBac vector (see below).
Mouse HA-tagged HOIP WT and CS (C693S/C696S) mutant were cloned into pTY-EF1A-hygromycinR lentiviral vector (see below).
The CamK activity described [ 29] as crucial for ookinete development in the mosquito vector (see below) is likely to be associated with one of the enzymes in this group.
A homozygous RGK1 line for this 5XUAS rsGFP-GUS reporter was chosen as the parent strain for producing a library using the pRGK335 trap vector (see below for library construction).
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All the primers used for amplification were designed by inserting appropriate restriction sites which could be used for subcloning all the fragments from pGEM-T cloning vectors into mammalian two-hybrid vectors pM (bait) and pVP16 (prey) and other protein expression vectors (see below) in correct reading frames.
These scenarios incorporate a range of assumptions about the vectors (see below).
To boost the initial retention of vectors following transfection, 3 μg of in vitro transcribed ARCA capped/polyadenylated EBNA1 mRNA was cotransfected with the vectors (see below).
With the exception of early-generation adenoviral vectors (see below), that retain some residual viral genes in their backbone, all other vector types are devoid of viral genes.
For the other experiments, 24 h after transfection cells were transduced with adenoviral vectors (see below) for the next 48 h.
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CEO of Professional Science Editing for Scientists @ prosciediting.com