Sentence examples for vector screening from inspiring English sources

Base calling, quality clipping and vector screening were performed with pregap4, which is part of the Staden sequence analysis package [69].

Sequencing of 3264 cDNAs from the 5' end yielded 3020 sequence reads, of which ∼34% (1019) shared no significant similarity to mitochondrial or bacterial genomes, and met a 70-bp length threshold after end-trimming and vector screening.

Vector screening was carried out using the crossmatch.

Vector screening and low quality sequence removal was done using the program Lucy [ 46].

Vector screening was performed using the Cross_match program with Codoncode InterPhace software.

Base-calling and vector screening were performed on the 4,977 obtained chromatograms using Phred, version 0.020425 [ 53].

All quality trimmed and vector screened 454 and Illumina reads were assembled using Velvet version 1.1.

The vector screened sequences were subjected to EST trimmer [ 70], to trim poly-A ends and low quality sequences.

Pre-miRNA fragments were then inserted into the XhoI site of a ΔNef shuttle vector, screened for orientation and then moved into the full length pNL4-3 provectorvector to produce the NL4-3 miR clones.

A total of 11,445 vector screened and clipped shotgun sequences, with Phred quality scores, were assembled using Phrap resulting in eight contigs ranging in size from 2 kbp to 158 kbp.

Finally, each synthetic hCR gene was independently subcloned into pEXP3-DEST vector and pBAD-DEST49 vector, for screening different expression systems.