Sentence examples for vector ratio of from inspiring English sources
The mutant inserts were ligated to the digested vector, using T4 DNA ligase with an insert to vector ratio of 4 1.
After treatment with 5 u of Tma endonuclease V, a ligation reaction with dephosphorylated pUC19 digested with AatII and PstI was set up using a molar insert to vector ratio of 7 1.
The SLiCE reaction was performed for 60 min at 37 °C with an insert:vector ratio of 1 1 and 3 1 for Prx IIE and G6PDH1, respectively Next, the effect of short homology length on cloning efficiency was evaluated using PCR fragments containing overlaps of varied lengths (Table 2).
In agreement with our data, previous results obtained with different ratios of Sema3A- and VEGF-expressing AAV showed that the anti-angiogenic effect was lost at a Sema3A VEGF vector ratio of 1 100, whereas NEM recruitment was still effectively increased (Zacchigna et al, 2008).
DNA was eluted in 30 μL deionized H2O and ligated into the pTZ57R/T vector using the InsTAclone PCR Cloning Kit (Fermentas), using an insert to vector ratio of 10too 1. Ligation products were transformed into NEB 5alpha competent E. coli (High Efficiency) (New England Biolabs) and plated on ampicillin LB plates.
We also observed a high cloning efficiency (at the optimal insert:vector ratio) regardless of the purification steps used.
Values of zero motion vector ratio, mean size of motion vector, uniformity of the movement, horizontalness of movement, and greenness are the classification parameters which are used to segregate the set of videos into different content classes.
These results suggest that both vector backbone concentration as well as gene insert to vector ratio are determinants of yeast MVT frequency.
Both factors together (large mPB Cre vector ratio and lack of episomal cassettes-derived fluorescence) evidence that CLoNe reliably labels clones through piggyBac mediated transposition.
A sharp principal scattering peak at a wave vector q* and several higher-order reflections appear, with wave vector ratios q/q* of 1 2 3 4.
Ligation was carried out using T4 DNA ligase at an insert: vector molar ratio of 3 1.
