Sentence examples for vector pro from inspiring English sources

The destination vector Pro UBQ10 :GW-RFP was generated by replacing the Pro 35S promoter with Pro UBQ10 using the primer pair ZP510/ZP511 with the SpeI/ HindIII double digestion sites from a previously described destination vector [ 55].

The experiment was designed as four groups: test group (pGL3-pro-UTR 5′ → 3′), Con-1 group (pGL3-pro-UTR 3′ → 5′), Con-2 group (pGL3-pro-UTR 5′ → 3′ del), and Con-3 group (empty vector pGL3-pro).

Transfection of an expression vector coding pro-IL-1 β into MC/9 cells induced the expression of pro-IL-1 β without expressing NLRP3, and doxycycline treatment induced the expression of wild type (WT -NLRP3 that WT -NLRP3d withatGFP (wasNLRP3-tagged-MC/9).

Co-transfection of Drosophila cells with pMT/V5-His/lacZ, lacZ-specific dsRNA and an HC-Pro expression vector (pMT-HC-Pro) resulted in staining of ~46% of cells, indicating that RNAi was being suppressed.

Briefly, dual luciferase reporter vectors pGL3-pro and control plasmid pRL-TK (Promega, USA) were used for the assay.

Moreover, temperature can increase the number of blood meals pro vector during each cycle amplifying significantly the level of transmission [ 56].

A single protein band corresponding to the β-mannanase was observed, whereas no protein band was observed in the supernatant from either S. lividans 1326 wild-type strain or S. lividans 1326/pUC702-pro strain, which is a transformant harboring the pUC702-pro vector as a control (Fig. 4a; Additional file 1: Table S1).

We further show symmetry between PBS and the primer by the ability of the synthetic tRNAB2 to confer escape from EC repression of a PBS-Pro vector.

The PCR product was digested with Kpn I/Avr II and ligated with the Kpn I/Nhe I-digested pSAK-35S-Pro vector to generate the mini binary vector pBD003 of 4,141 bp. Figure 1 Construction of the pBD003 binary vector.

We cloned them into pGL3-pro vector, respectively.

NIH 3T3 cells were transfected with expression vectors using Metafectene Pro (Cambio, Cambridge, UK) according to the manufacturer's instructions.