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A similar vector, CBβ-Gal, instead of IFN-γ was used to prepare the clonal producer line DA/CBβ-Gal, which produced a vector preparation with a titer of approximately 5.5 × 106 cfu/ml on HT-1080 cells.
HR and pathogenicity-associated 1. the empty vector preparation.
The empty vector preparation (EVP) was produced by using the vector without genetic combination.
However, the capsid proteins that are an essential component of the input viral vector and any residual helper virus in the vector preparation could induce an immune response.
The empty vector preparation (EVP) and the Hpa1-His or Hpa110 42-His fusion protein preparation were purified by nickel cHpa110 42-His and elution with aqueous imidazole solutions.
Vector preparation is the major factor impacting cloning efficiency.
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The pIbCP-LK-LK vector preparations were directly injected in the lactating goat mammary glands.
Approaches supporting improved productivity or quality of vector preparations are discussed, mainly focusing on medium design and genetic manipulation.
High-titer (paramagnetic) retroviral vector preparations can be used for magnetic field-dependent retroviral infection in vitro.
Important advantageous properties of adenoviral vectors include: feasible production of high-titer vector preparations, high efficiency in transducing both quiescent and actively dividing cells, high levels of transient gene expression, and a lack of mutagenic properties associated with integrating vectors.
Purification, dialysis, and titration of the vector preparations via real-time PCR were performed, averaging 1010 transgene copies/ml with approximately 1/500 functional recombinant viral particles (Cucchiarini et al. 2011; Frisch et al. 2015b; Rey-Rico et al. 2015).
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