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(A) Neuro2a cells transfected with control vector (Vector) or vector harboring TET1, TET2, or TET3 gene were co-immunostained with Flag and GAP43 antibodies.
(B) Neuro2a cells transfected with control vector (Vector) or vector harboring WT TET1 (TET1), TET1 dCXXC (dCXXC), and TET1 CD mut (CD mut) were lysed and immunoblotted with TET1 antibody.
(D) Neuro2a cells transfected with control vector (Vector) or vector harboring WT TET1 (TET1), TET1 dCXXC (dCXXC), and TET1 CD mut (CD mut) gene were co-immunostained with Flag and GAP43 antibodies.
(C) The 1 μg of myc-tagged empty vector or vector with DSS1-myc was radiolabeled with L-[35S]-methionine (10 μCi) in an in vitro TNT cell-free protein synthesis system, and incubated overnight at 4°C with HeLa lysate in the absence or presence of Bortezomib (20 μmol/L).
HEK293T cells expressing MEK1-ED without or with NdpA (D); HEK293T cells transfected with empty vector or vector encoding flag-tagged NdpA were treated with 100 ng/mL of EGF for 15 min (E); A549 cells were infected with WT or NdpA-overexpressing K. pneumoniae (NdpA-KP) for indicated time (F).
PPARA promoter-reporter constructs were cotransfected with empty expression vector or vector expressing ERRα or ERRγ.
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293 cells were transfected with the pCGN-HAM empty vector, or vectors expressing HA-EAF1 or HA-EAF2/U19.
The cells were transfected with combinations of an empty vector or vectors encoding HuUbE1L, HuUbE1 and UbcH8 together with FLAG-tagged HuISG15, AgmISG15 or MoISG15.
It is also not known the optimum dose of each vector or vector-loaded gene concentration that can enhance mandibular growth without inducing unnecessary overgrowth of the mandibles or inducing neoplastic growth.
Mice were either treated with the Blank expression vector or vectors expressing IFN subtypes (IFNA6, A9 and B) or the gB transgene alone or in combination.
The arthropod vector or vectors, and vertebrate reservoir or reservoirs of E. chaffeensis in Costa Rica are unknown, and further ecologic studies are required to determine these aspects of human monocytic ehrlichiosis in Central America.
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