Sentence examples for vector or short from inspiring English sources

Cells were plated onto 10-cm dishes, maintained in antibiotic-free medium for 24 hr, and then transfected for additional 24 hr before treatments with a mixture containing Opti-MEM, 8 μL/well LipofectAMINE 2000 (Invitrogen, Milan, Italy), and 0.5 μg/well vector or short hairpin GPR30 (shGPR30) (Albanito et al. 2008), control small interfering RNA (siRNA), or ERα siRNA (Sigma-Aldrich).

Cortical neurons were infected with lentivirus encoding FUGW empty vector or TC10 short hairpin RNA construct (shRNA) on DIV7 and lysed for real-time PCR on DIV11.

To obtain pre-adipocyte clones with long term down regulation of DNA-PKcs expression, the 3T3F442A cells were transfected using Amaxa electropation system (Nucleofector solution V; program: T030) with EBV-based vectors containing either control or short hairpin RNA coding sequences for murine DNA-PKcs as previously described [22].

Primary neurons were transfected with lentiviral vector encoding ALDH2 (LV-ALDH2) or short hairpin RNA (shRNA) targeting ALDH2 (sh-ALDH2).

KPC cells were transfected with 1 μg of MISSION® pLKO.1-puro empty vector control plasmid DNA (EV) or short hairpin targeting LOX-containing plasmid (shLOX) (Sigma) using Lipofectamine (Invitrogen) for 48 h.

Control pGL3 reporter vector or pGL3 containing the short portion (s-UTR) or the full length (UTR) p27-3'UTR were transfected in QMb-ts myoblasts and luciferase activity was measured both in myoblasts and in myotubes (Fig. 6A).

We over-expressed OS-9 or attempted to reduce OS-9 levels either by transient transfection of short interfering RNA or short hairpin vectors.

In addition, they are transduced with control or retroviral vector expressing short hairpin RNAs (shRNA) specific for mouse C7ORF41.

To study interactors of Oxr1 (both full length and short isoforms), N2a cells were transfected with the HA-tagged Oxr1 full length or short isoform in pCX vector.

Several RNAi studies performed in human tumor cell lines using synthetic small interfering RNAs (siRNAs) or vector-based short hairpin RNAs (shRNAs) targeting defined gene families or genome-wide libraries have identified modulators of drug sensitivity [ 27- 33].

The removal of vector and short length (20 bp or less) sequences resulted in 31,485 high-quality ESTs with an average length of 531 bp.