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Primers annealing to pComb3X vector or heavy and light chain constant regions (Table S1 and S2) were used to amplify the scFvs from the gDNA antibody libraries and Fds and light chains from the cDNA antibody libraries.
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*targeted/neor clones To evaluate the homogeneity of expression level of the daughter cell clones, three master cell clones were targeted with expression vectors encoding eGFP, RFP or heavy and light chains of an antibody.
Environmental factors, including human activities, that enhance population densities of vector mosquitoes (heavy rains followed by floods, irrigation, higher than usual temperature, or formation of ecologic niches that enable mass breeding of mosquitoes) could increase the incidence of West Nile fever.
Control cells (pBabe plasmid empty vector) were labeled light while Flag-His ISG15 ectopic expression cells (pBabe-Flag-His ISG15 mature) were labeled medium or heavy.
This design of a single cassette vector combining heavy and light chain expression elements allowed rapid production and secretion of correctly processed and assembled intact immunoglobulins from recombinant baculovirus infected insect cells.
There are no robes or heavy beards.
Or heavy and trepidatious?
For proteomics analysis, whole cell extracts isolated separately from "light" (empty vector) and "heavy" (eIF6 over-expression) cell lines were mixed in equal amounts.
In order to circumvent these problems, single domain heavy chain cDNAs were subcloned into a mammalian expression vector and heavy chain antibodies (HCAbs) made up of variable heavy chain regions fused to mouse Fc region were generated.
Greasy or heavy makeup [8].
Is it light or heavy?
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