Sentence examples for vector open from inspiring English sources

For the construction of the Cas9-GFP expression vector, open reading frame encoding Cas9-NLS was amplified by PCR from pSLQ1658-dCas9-EGFP22 (obtained from Addgene).

The shRNA expression vectors for knockdown of mouse Etv5 and Maf were generated using MSCV-LTRmiR30-PIG (LMP) vector (Open Biosystems) according to the manufacturer's instruction.

The library was complemented with additional 40 shRNAs in pLKO lentiviral vector (Broad Institute TRC library, MISSION TRC-Hs 1.0 library; Sigma, St Louis, MO, USA) and with 43 shRNAs in pGIPZ mir-30-based vector (Open Biosystems, Fisher Scientific, Vantaa, Finland).

Examining n fuzzy sets A 1,A 2,…, A n defined on support X, which have GoM a j (x) Open image in new window, and a weight vector Open image in new window satisfying the condition Open image in new window, weighted mean h w may be is defined as: Open image in new window (4).

Complementary sequences were annealed and the resulting double-stranded DNA was ligated to Xho I/Not I-digested pLemiR™ vector (Open Biosystems®, Huntsville, AL).

For depletion of PKD1 and 3, hairpins were cut from the pSM2 Retroviral shRNAmir libraries (Open Biosystems, Huntsville, USA) and cloned into the pGipz vector (Open Biosystems).

This new vector opens up opportunities in fish DNA vaccinology and in the production of fish recombinant proteins.

The data-dependent component was eliminated from this vector, opening way to easily obtain an initial CIR estimate.

After restriction enzyme digestion by SacI and MluI, both fragments were ligated to pMir-Report vector opened with SacI and MluI.

After double restriction enzyme digestion by SacI and MluI, both fragments were ligated with pMir-Report vector opened with SacI and MluI.

Digesting the PCR generated donor fragment with 'A' and 'b' enzymes, and ligating to the recipient vector opened up with 'a' and 'B' enzymes, creates a nascent vector where each of the original 'b', 'a', 'and and 'B' sites are destroyed.