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In parallel, mock-transfected cell clones were also developed bearing empty vector only, as well as mutant clones containing a putative PTPase-dead mutant Tensin3 (Tns3 Mut) cDNA.
psittaci infection of mammalian cells is somehow connected with chlamydial IncA, we transfected HEK293 cells with a mammalian expression vector containing the HA-tagged codon-adapted cytoplasmic domain of IncA (aa 121-383) from Cp. psittaci, or the vector only as a control.
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However, since we use the vectors only as templates for PCR linearization the amount of DNA used for a single PCR is very low and usually sufficient for multiple SLIC reactions.
However, virions from these cells were just as infectious as virions released from vector only controls, yet not as infectious as virions from cells either stably or transiently-expressing CIITA (Figure 2D).
A375 cells stably overexpressing murine bcl-2α (A375/mbcl-2 A375/mbcl-2randfected with vector only (A375/pIRES) as contransfectedreated for 24 h with 100 μ M pamidronate.
In addition to the JAK2/STAT-1/p21/Cdk2 patheay, the proliferation capacity of NRK-52E transfected with WT, R742X and 1 702 PKD2 appeared unaltered compared to vector only transfectants as judged by PCNA Western blot analysis.
The fold changes of steady-state RNA levels in ank-transduced cells compared with cells transduced with empty vector only were determined as previously described.
Beyond sharing HBV's hepatocyte tropism, such a vector would be self-renewing, but only as long as wild-type HBV is present.
As expected, the vector only cell line was incapable of restoring EGFP fluorescence.
The EV GFP vector only produced GFP.
i. Vector only (GFP).
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