Sentence examples for vector mixed with from inspiring English sources

In contrast, control animals treated with AAV vector only or vector mixed with SVP[empty] showed a boost in antibody titers against AAV8 and no significant increase in SEAP transgene expression after the second injection (Fig. 1g, h).

Cells were transfected in duplicate with 2 μg Ngn2-, Mash1-, or empty pCAGGS vectors and 0.1 μg GFP control vector mixed with Lipofectamine 2000 (Invitrogen) following the manufacturer's recommendations.

The cell pellet was resuspended in the vector solution mix consisting of 2 μg of expression vector mixed with 100 μl of the supplemented Nucleofector™ Cell Line SG solution.

In this case, animals (n = 10 per group) received a 4 µl injection of a LacZ lentiviral vector mixed (ratio 2∶1) with a vector encoding the wild-type N-terminal Htt fragment (LacZ+Htt171-18Q) or expanded Htt (LacZ+Htt171-82Q) inthethe left and the right striatum, respectively.

To obtain transgenic 10UAS-Cas9 flies, the PiggyBac-10UAS-cas9 vector was mixed with helper vector PiggyBac-transposase (Thibault et al. 2004) and injected into w fly embryos.

To obtain transgenic vasa -Cas9 flies, the PiggyBac- vasa -cas9 vector was mixed with helper vector PiggyBac-transposase (Thibault et al. 2004) and injected into w fly embryos.

These reporter plasmids, TOPflash (Millipore), or empty pGL3-Vector were mixed with pRL-TK vector (Promega) at a 10 1 ratio, and transfected to P19 embryonic carcinoma cells (ATCC) using lipofectamine 2000 (Invirogen).

200 ng of the linear vector was mixed with insert DNA at equal molar ratio in a 20 µl volume containing Phusion DNA polymerase reaction mixture (Finnzymes).

For each dish, 10 µg of the lentiviral vector were mixed with 3.5 µg of the VSV- G envelope plasmid and 6.5 µg of the packaging plasmid (pMD2.VSVG and pCMVΔR8.74, respectively [29]).

A 4.9 Kb linear fragment containing T2/OncZ was released from the plasmid vector and mixed with a linear fragment containing a widely expressed ß-actin:RFP reporter cassette [36] before injection (Fig. 1 A).

MMP-1 or MMP-2 vector was mixed with FuGENE HD transfection reagent (Promega).