Sentence examples for vector mix from inspiring English sources

The vector mix was introduced by microparticle bombardment using a Biolistic PDS-1000/He PDS-1000/Helivery System (Bio-Rad, Hercules, CA, USA).

The jetPEI cations mixture was then added to the transfection vector mix and left for 30 min at room temperature.

16 Lentivirus was made by transfection of the 293T producer cell line with the lentiviral vector and packing vector mix (System Biosciences).

A total of 5-8 μl of the digested vector mix, typically containing 100 ng of DNA for each vector, was used per transfection.

Indeed, we could detect by immunohistochemistry a clear overexpression of both mouse PGC-1α and human aSyn in a significant proportion of TH-labeled dopaminergic neurons, 6 months post-injection of the vector mix.

Aliquots of 2 µl of ligation product were incubated at room temperature for 5 min with 3 µl of StrataClone Cloning Buffer and 1 µl of StrataClone vector Mix.

A generative model of ICA is linear,, where,, and are the observed data vector, mixing matrix, and source vector, respectively.

The cell pellet was resuspended in the vector solution mix consisting of 2 μg of expression vector mixed with 100 μl of the supplemented Nucleofector™ Cell Line SG solution.

Cells were transfected in duplicate with 2 μg Ngn2-, Mash1-, or empty pCAGGS vectors and 0.1 μg GFP control vector mixed with Lipofectamine 2000 (Invitrogen) following the manufacturer's recommendations.

"The likely hotspots [for outbreak] are Southeast Asia, South Asia, West Africa and South America where the combination of potential vectors, mixing of humans, livestock and wildlife and less capacity for public health surveillance are likely to co-exist," Hill-Cawthorne says.

"The likely hotspots [for outbreak] are Southeast Asia, South Asia, West Africa, and South America where the combination of potential vectors, mixing of humans, livestock, and wildlife, and less capacity for public health surveillance are likely to coexist," Hill-Cawthorne said.