Sentence examples for vector materials from inspiring English sources

To address the function of KLF13 in the early stage of porcine adipocyte differentiation, we established three different small interference RNA (siRNA) sequences against KLF13 and constructed KLF13 overexpression vector (Materials and methods).

We cloned the same engineered and native FtsZ, NS5B and P38α genes into a CFS expression vector (Materials and Methods) ensuring that the full length proteins had identical amino acid sequences.

The issue of systemic spill over of vector material into the cardiac atria and thereafter into the systemic circulation would need to be evaluated by sampling local and distant organs.

Subsequently, we expressed HPV-16 E7 in HaCaT keratinocytes via the pLXSN retroviral vector (see Materials and Methods).

Two BP reactions were set between the expression clone and either an attP3/P5 (Fig. 7A) or an attP1/P2 cassette (Fig. 7B) purified from the corresponding donor vector (see materials and methods).

The exact positions of the insertions were determined by PCR and sequencing using Lrsam1 forward and reverse primers in the gene trap vector (see Materials and Methods).

Some cell lines were stably transfected with red fluorescent protein using a dsRed-expressing lentiviral vector (Supplemental Materials and Methods).

In both cases, we generated template DNA by cloning the appropriate PCR product into a cloning vector (see Materials and Methods).

As shown in Fig. 8, panel A, Nrf2 gene expression (as reflected by mRNA levels) was decreased by approximately 60% (p < 0.05) using the silencing vector (see Materials and Methods).

To identify factors that stimulate Brn-3b promoter activity and therefore gene expression in breast cancer cells, the BSX reporter construct, containing the putative Brn-3b promoter and regulatory sequences cloned into pGL2 basic reporter vector (see Materials and methods, and Figure 1b) was used in transfection studies.

It is further assumed that the constitutive relation is differentiable function of the position vector of material particles in the stress free reference configuration.