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For each mutation, the purified extracts were mixed with the pMD19-T simple vector master mix (Takara BIO) according to the manufacturer's instruction and ligated at 16°C for 2 hours.
Similar(59)
where x ( t ) = ( x 1 ( t ), x 2 ( t ), …, x n ( t ) ) T ∈ R n and y ( t ) = ( y 1 ( t ), y 2 ( t ), …, y n ( t ) ) T ∈ R n are the state vectors of the master system and the slave system, respectively.
Triplicate reactions were performed for each transformant.> -wrap-foot> Underegionsregindicateicate restriction enzyme sites The master Ti vector pPK2BarGFPD [ 23] containing the phosphinothricin resistant bar marker, was employed to test transformation efficiency of M. thermophila mediated by A. tumefaciens.
Hence, persons without the skill TOOL in their person-skill-vector will fail to master this particular task, but also all other tasks which require this particular TOOL-skill.
The master receives all slave vectors and, from these vectors and the identifier the slave has sent, it builds the similarities matrix.
The approach adopts a hybrid vector which includes measured data at master degrees of freedom (dofs) and constant values at slave dofs.
In this approach, fingerprint of receiver is sent to sender and fingerprint of sender is used as cover image and the watermarked image containing the master key and random vector is sent to receiver.
The final PCR product was gel purified and subsequently cloned into an Entry vector, pDONR221 (Invitrogen), to generate a master clone.
During the matrix lines calculation, each slave stores the results of all lines in a single vector (SimVect) which is sent to master.
u i is the nodal displacement vector that includes one slave node and the master segment nodes.
*targeted/neor clones To evaluate the homogeneity of expression level of the daughter cell clones, three master cell clones were targeted with expression vectors encoding eGFP, RFP or heavy and light chains of an antibody.
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CEO of Professional Science Editing for Scientists @ prosciediting.com