Sentence examples for vector kind from inspiring English sources

A 1527 bp genomic DNA upstream of the agr-1 ATG start codon was amplified with the primers XY1 and XY2 and cloned into the PstI-XbaI sites of pVH20.01 (promoterless yfp vector), kind gift for Prof. Harald Hutter.

CDK11p58 and CDK11p58N were amplified by PCR using the oligonucleotides 5'TandATTCCCAGTGAAGATGAAGAACG3' and 5'TandATCCCCAGTGAAGATGAAGAACG3' and subcloned into the EcoRI and BamH1 sites of the COM263 pcDNA3.1/3xmycA/TO vector (kind gift of Pr Erich Nigg, University of Basel).

For complementation of the Xcv 85* ΔxopD mutant strain, XopD1-760 and XopD216-760 werecombinedned into a pLAFR6-GW-3HA vector (kind gift of Laurent Deslandes, LIPM, Castanet-Tolosan, France) that allows expression of HA-tagged XopD proteins under the control of a constitutive lac promoter.

To produce AAV, the ChR2-YFP coding region was blunt cloned into the AAV shuttle vector (kind gift of Sandy Kuhlman, [19]) containing a transcriptional insulator flanked by two loxP sites (loxP-STOP-loxP - LSL) downstream of the cytomegalovirus (CMV) promoter.

The PCR product was then subcloned in frame with GFP into pARL vector (Kind gift of the Dr C. Sanchez Heidelberg, Germany) [ 47] digested with XhoI and KpnI.

Human STEAP, STAMP1, STAMP2, and STAMP3 cDNAs were cloned into SR-alpha vector (kind gift from Tiliang Deng) to introduce an N-terminal HA tag.

The choice of gene delivery strategies among several delivery systems depend on some factors including the improvement of vectors, kind of expression systems, and better understanding of molecular biology of target site and employing of the advances in the identification of new genes and new targets [11].

All the lentiviral shuttle vectors and helper vectors were kind gifts from David Baltimore.

C2C12 cells were electroporated together with 0.4 μg of a BMP-2 expression vector (a kind gift from Dr. Katagiri) and 0.1 μg of pCMV-Tag, which expresses a neomycin-resistance gene.

The retroviral vector LXSP (kind gift from A.G. Rolink, Basel) was used to generate the double-reporter vector.

Plasmids containing c-Src gene mutants and pEVX vector were kind gifts from Dr. David Shalloway and Dr. Michael Botchan, respectively (Addgene, Cambridge, MA).