The cell lysates (Cell) and culture supernatants (Sup) of 293T cells transfected with either wild type (WT) or CASP3*-gag-pol (CASP3*) expression vector were analyzed by Western Blot.
By means of computerized image analysis the angles of the cells long axis with respect to the acceleration vector were analyzed in order to calculate and compare the reorientation kinetics of the immobilized cells versus that of the controls.
The scene durations were normalized as in the previous studies and the scene-duration vector was analyzed using the exact local Whittle estimator, as in Studies 2 4.
Cell lysates prepared from cells transfected with VerUTR or the control vector were analyzed for Rb1 expression by western blotting.
The functionality of the loxP sites in the final targeting vector was analyzed by appropriate restriction digests after transformation into the Cre-expressing E. coli strain 294-Cre [29].
One of the clones (in a pPAC4 vector) was analyzed by Southern blotting with Fmo5 exon-specific PCR products as probes.
In addition, MCF-7 cells transfected with pRSV-hPit-1 vector were analyzed by quantitative PCR at 24, 48, and 72 h (Fig. 1C).
The dependence of the expression levels of the metagene probesets on the dataset vector was analyzed using the Kruskal-Wallis statistic (Additional file 4, Supplementary Methods Section 3).
