In a subsequent study, C57BL/6 -gld/gld mice, which are Fas ligand deficient, were treated with an adenoviral viral vector inducing the overexpression of Fas ligand [ 144].
It is possible that two identical insulator sequences separated by an appropriate spacing as in the d2 × 250 bp.CMV vector and the reversed transcribed form of the 1.2 kb.CMV vector induce the formation of secondary structure incompatible with proper transduction and transgene expression.
As described above, the cloning strategy based on Gateway entry vectors induced the addition of attB sites (48 nucleotides) in-between the original vector ATG (within the NcoI site) and the initiation codon of the cDNA (Figure 1B).
We conclude that antisense U7 snRNAs delivered by Adv-5 vectors induces the expression of functional SMN protein in cell cultures.
The results showed that all three gene-delivery vectors induce the formation of tubulovesicular autophagosomes (TVAs) rather than classical autophagosomes.
The corresponding vector induced consistent staining in the neural tube (71% embryos, n = 7, p = 8*10−5) and brain (57%, n = 7) (Figure 3b).
In this study, we found that the VIGS method using the "one-step" BPMV vector induced efficient silencing of the endogene PvPDS in common bean cv.
In SK-OV-3 ovary cancer cell line, overexpressing about 60 times the erbB-2 transcript, only the p756-LUC vector induced a significant increase in the transcription level.
