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The product was subsequently cloned into the pET-15b (Merck/Novagen) expression vector for production of protein with an N-terminal His tag.
To clone the virB12 gene in pET28a expression vector for production of recombinant protein to be used as antigenic component for future serological test development.
As a neuroprotective strategy, these cells were then co-cultured across a membrane insert with mesenchymal stem cells (MSCs) engineered with a lentiviral vector for production of BDNF (BDNF-MSCs).
We report about the establishment of transgenic cell lines that over-express human CCN2/CTGF and an adenoviral expression vector for production of rat CCN3/NOV.
A triple-Flag tag sequence was cloned in frame to the 3' end of the full-length cDNA and inserted into an AdEasy shuttle vector for production of recombinant adenovirus.
In order to generate a vector for production of TEVp, a KpnI-site that should facilitate the replacement of TEVp with other proteases of interest was first introduced upstream of the TEVp coding sequence in pMal-TEV2.
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The pARK and pDRK plasmids were tailored for expression of GvpC fusion proteins and represent convenient vectors for production of bioengineered GVNPs.
The ORFs were cloned into an expression vector pDEST17-D188), for production of proteins with an N-terminal 6xHis-tag.
Potential tumour associated antigens derived from SEREX screens were isolated and sub cloned in the expression vector pQE30NST for production of His-tag (histidine-tagged) fusion proteins [ 16- 20].
We have constructed a dual expression vector for the production of recombinant proteins in both Escherichia coli and insect cells.
We have developed a Cucumber mosaic virus (CMV -based expression veCMV -basedhexpressionon of heterologous proteins in plants.
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