Sentence examples for vector for overnight from inspiring English sources

Ligation was performed in 20 μl reaction volume using 400 units of T4 DNA ligase (New England Biolabs) with 2 μl of PCR fragments and 15 to 20 ng of prepped vector for overnight at 16°C.

The solubilised fraction can either proceed to western blot analysis or incubate with WGA beads (Vector Labs) overnight at 4°C for glycoprotein enrichment.

HL-1 cells on 35 mm dishes (1 × 10/well) were transfected for overnight with pGL3/SAP97, pGL3/ΔHSEs or the parental vector.

When double-immunostaining for VGF/GFAP, VGF/NeuN, or VGF/CD11b were performed, these antibodies were applied to the sections for overnight at 4°C after blocking with 0.01 M PBS containing 10% normal goat serum (Vector) or mouse-on-mouse blocking reagent (M.O.M. immunodetection kit, Vector) for 1 h.

For immunohistochemical analysis, ileum frozen tissues incubated with primary antibody for overnight at 4 °C were treated with biotinylated secondary antibody solution (Vectastain Elite ABC kit, Vector Laboratories, CA, USA) for 1 h at room temperature.

A single colony of GS115-SAM2/control GS115-SAM2/controlwith vector alone, GS115 on YPD agar platransformedculated in 5.0 ml of YPD medium and growitht 30°C, 280 rpm for ovectorht, aloneas used as inoculum.

Central time for overnight delivery.

Plates were incubated at 37°C for overnight.

The mixture was further incubated for overnight.

Again the plates were incubated at 30°C for overnight.

The bright yellow powder was dried in oven for overnight.