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The authors concluded that Fab fragments were not an optimal vector for imaging due to their rapid clearance, low accumulation in tumor and high renal accumulation.
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The 68Ga has a very favourable positron emission (1880 keV, 90%) for PET imaging, and a short half-life (68 min) suitably matched for imaging with peptide vectors.
The name [ib] stands for imaging bridging, imaging browsing and image brightening.
We would like to thank Dr. Ilze Matise (Masonic Cancer Center Comparative Pathology Core) for the initial pathology consults, Dr. Jeff Rosen (Baylor College of Medicine) for the gift of the WkbpAII transgene vector, Abby Olsen for transgene preparation, and Jonathan Henriksen (University of Minnesota BioNet Digital Imaging Facility) for imaging assistance.
However, because the clock signals were only intended for imaging depths of 2.9mm and 3.7mm (for the 840nm and 1040nm systems, respectively), the recalibration vector was first interpolated to increase the achievable imaging depth to 9.4mm and 12.4mm, respectively.
Cells were transfected with siRNA oligos or plasmid vectors for 48 or 24 h, respectively, prior to imaging and transferred to Leibovitz's L15 medium (Invitrogen) for imaging at 37°C.
Cells were transfected with siRNA oligos or plasmid vectors for 48 or 24 hr, respectively, prior to imaging and were transferred to Leibovitz's L15 medium (Invitrogen) for imaging at 37°C.
Portable data for imaging.
Specific advice for imaging children.
We thank A McCauley for imaging assistance.
Sections were mounted in mowiol for imaging.
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