To facilitate functional analysis, cDNAs were introduced in a Gateway-based cloning vector for high-throughput functional analysis of genes in planta.
With the goal of developing this vector for high-throughput VIGS studies in common bean, we optimized the conditions for rub-inoculation of infectious BPMV-derived plasmids in common bean cv.
The PCR product was cloned into the AlwNI-XhoI-digested pET-31b vector (Novagen), a vector for high-level expression of peptide with an N-terminal KSI (ketosteroid isomerase) tag.
With the goal of adapting the 'one-step' BPMV vector for high-throughput VIGS studies in common bean, we first aimed to optimize the conditions for rub-inoculation of infectious BPMV-derived plasmids in common bean cv.
Given the popularity and efficiency of the pBABE vector system for retroviral transduction and overexpression of cDNAs, we believe that the pBLIC version will simplify and extend its applications, for instance by making it a feasible vector for high-throughput cloning applications such as generation of cDNA libraries.
The article entitled "Progressive refinement of beamforming vectors for high resolution limited feedback" by R. W. Heath et al. proposes to use a progressively scaled local codebook to enable high resolution quantization and reconstruction for multiuser MIMO with zero-forcing precoding.
To facilitate future functional analyses of all LRR-RLKs, we generated 4 different GatewayR-compatible binary vectors for high through-put cloning of LRR-RLKs.
In the present effort, we have developed such a platform, which consists of a series of ligation independent clone (LIC) based vectors, for high throughput cloning and expression screening and then tested the platform with 41 putative integral membrane proteins from Mycobacterium tuberculosis.
In conclusion, we have established a versatile vector toolkit for high efficiency and throughput gene functional analysis in rice.
Goda, T. Tenno, H. Takasu, H. Hiroaki, M. Shirakawa, The PRESAT-vector: asymmetric T-vector for high-throughput screening of soluble protein domains for structural proteomics, Protein Sci. 13 (2004) 652 658], which facilitates unidirectional cloning of any PCR-amplified DNA fragments corresponding to the protein of interest.
This chapter discusses the molecular architecture of non-viral vectors for high-level protein production.
