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We subjectively selected the Eigen genes having angles of less than 15° to any condition vector for further inspection (italics in Tables 1, 2).
All inserts isolated were further subcloned into the pFB vector for further validation of their capacity to transform.
The amplified PCR product was confirmed by sequencing and cloned into T4 vector for further manipulation.
Thus, we selected the pTP-mi5 expression vector for further optimization.
The resulting cDNAs were cloned into the pENTR1A vector for further recombination into the PINCO vector as described in Beissert et al. 2008 [ 13] using the Gateway LR-clonase enzyme kit (Invitrogen, Karslruhe, Germany).
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Feature usage values are often times stored permanently in a database and/or transformed into vectors for further analysis utilizing statistical techniques.
The primary vpr alleles were subcloned into expression vectors for further characterization.
We chose the most promising vectors for further experiments in a larger group of animals (n = 5 mice per clone).
The construction of vectors for further generation of P0 baculovirus stocks through Sf9 cells transfection was performed as follows: the DNA sequence of H1N1 A/Puerto Rico/8/1934 HA (AB671289.1), NA (AB671290.1) and M1 (CY033578.1) were obtained from NCBI's influenza database.
Each reference vector is used to create a bundle of vector lines for further locating the centers of holes.
This vector was used to introduce the Ezh2 coding sequence into pLenti6/V5-DEST by recombination to generate the transfer vector pLenti6-Ezh2 (furtherther details see Invitrogen's ViraPower Lentiviral Expression System manual).
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