Sentence examples for vector for display from inspiring English sources

RNA isolation and library constructions were performed as previously described amplifying the VHH genes and ligate them into a phagemid vector for display on phage resulting in libraries of size around 108 [8].

In the present study, we applied a novel pKM19 vector for display of recombinant antibodies in single-chain format.

To improve the stability of the wild-type scFv molecule by alteration of the linker peptide, this region was randomised by PCR and the resulting scFv fragments were recloned into a phagemid vector for display on phage.

To examine the possibility of using the humanised scFv antibody as a template to select entire human fragments from phage display libraries by chain shuffling (Marks et al, 1992), both wild-type sequence scFv 4.10W and mutant 4.9M were recloned in a phagemid vector for display on phage.

We report the development of a novel phagemid vector, pKM19, for display of recombinant antibodies in single-chain format (scFv) on the surface of filamentous phage.

In recent years, the use of surface-display vectors for displaying polypeptides on the surface of bacteriophage and bacteria, combined with in vitro selection technologies, has transformed the way in which we generate and manipulate ligands, such as enzymes, antibodies and peptides.

The resulting mRNA coding for the heavy chain antibodies was isolated, transcribed to cDNA, and cloned into a phagemid vector for phage display library construction.

This study showed a potential utility of bifunctional lambda phages, but did not investigate the capacity of the lambda vector for double display of large proteins.

The resulting mRNA coding for the variable domain of heavy-chain antibodies (VHH) were isolated, transcribed to cDNA, and cloned into a phagemid vector for phage display library construction.

In our previous work [ 6] we designed a new-concept lambda vector for the display of cDNA-encoded protein fragments as fusion to the N-terminus of bacteriophage gpD, allowing us to overcome this obstacle.

As an alternative approach, in this study a new vector for cell surface display on Y. lipolytica cells was developed.