Sentence examples for vector for bacterial from inspiring English sources

An N-terminal fragment of OCRL1 encoding amino acids 1 303 was cloned into pGEX 4T-1 vector for bacterial expression of protein and antibody production.

NdeI/BamHI DNA fragments were ligated into pET15b-His(6) vector for bacterial expression of N-terminally His(6 -tagged human CaM (pMS6 -taggedCaM1–4m (pMS645).

ROCK-I1−406 ROCK-I1−406 ROCK-I1−406rminal His tag into the pFastBac system for the generation of baculovirus, and RhoE1−200 wase cloned into the pET28 vector for bacterial expression.

For crystallization, LepB (residues 1-618) were also cloned into the pQE-80L vector for bacterial expression, and proteins were further purified by Mono Q ion exchange and Sephadex 75 gel filtration chromatography.

cDNA constructs encoding human Arf1 and Arf6 were obtained from the cDNA Resource Center, Guthrie Research Institute, Sayre, PA, U.S.A. Full-length Arf1 (residues 1 181), Arf1 Δ N-terminus (residues 18 181) and full-length Arf6 (residues 1 175) were subsequently cloned into the pBac4×1 vector for baculovirus expression or the pET15b vector for bacterial expression.

Ticks in various regions of the world are vectors for bacterial, viral, and protozoal pathogens (5 ).

To determine whether parasitic helminths act as vectors for bacterial pathogens in human and animal hosts, the first step is to investigate whether parasitic helminths carry viable pathogenic bacteria.

This methodology has been used for PCR-based amplification of genomic DNA sequences [ 4], to generate vectors for bacterial protein overexpression [ 5], for high-throughput cloning of biocatalysts from prokaryotic genomes [ 6], for the addition of variable-length C-terminal histidine tags [ 7], and for gene silencing in plant cells [ 8].

The Antennapedia homeodomain (Antp) gene was subcloned from pA4C1 (Prof. Crisanti, Imperial College) into vector pDS56 for bacterial expression (pDS56Antp).

(18) Restriction fragments from vectors used for bacterial expression of the extracellular domains of three variants of human DC-SIGNR(19) were moved into a vector encoding an N-terminal His6 tag previously designed for use with DC-SIGN.

The pEPI-1 vector contains a total of 305 CpG motives, most of them within the vector elements necessary for bacterial propagation.