Sentence examples for vector expression and from inspiring English sources

Additionally, we highlight technologies to utilize these tools in vitro and in vivo, including Cre dependent viral vector expression and two-photon microscopy.

This study also shows that the CCD camera is a powerful tool to follow vector expression and IRES-dependent translatinn in live animals.

The use of this vector to introduce p19Arf into tumor cells that harbor p53wt should yield viral expression of p19Arf which, in turn, would activate the endogenous p53 and result in enhanced vector expression and tumor suppression.

Since p19Arf can work in conjunction with the p53 pathway, we proposed that introduction of exogenous p19Arf may functionally activate endogenous p53, impacting both vector expression and tumor cell proliferation.

We sought to target endogenous p53 wt to both drive vector expression and inhibit tumor proliferation by including the p19Arf cDNA in the pCLPG retrovirus, a vector that contains a p53-responsive promoter used to control expression of the transgene.

D.I. and M.C. performed all NT and characterization work of Prm cells, and M.C. performed the vector expressions and F.Z. did the embryological work.

We describe here protocols for the construction of bacterial LSm coexpression vectors, expression and purification of LSm proteins and subcomplexes, and the in vitro reconstitution of fully functional human LSm1‐7 and LSm2‐8 heptameric complexes.

Fig. 1 Recombinant HBD3 protein prokaryotic expression vector construction and verification.

MJPF carried out the vector construction and expression of GST fusion proteins.

This expression system includes vectors, expression cassettes and site-specific recombinases for the selective elimination of marker genes.

SCOOP also allows affordable screening in alternative vectors, expression strains and physical conditions, which is challenging in large-scale protein production programs.