Sentence examples for vector expressed from inspiring English sources

A synthetic gene encoding DT386-BR2 was cloned in pET28a expression vector, expressed and purified by affinity chromatography.

Constructs were cloned into pET 28a (Novagen) expression vector, expressed in BL21 cells and subsequently analyzed on sodium dodecylsulfate-polyacrylamide gels.

This was cloned into pGEX-4T-2 expression vector, expressed as a GST tagged peptide, purified as per standard protocol and this fusion protein (206 aa) was used to raise antibodies in rabbits at Thermo Fischer/Open Biosystems, Rockford, IL, USA (Ring et al., 2002).

The set of 24 peptides were randomly chosen, inserted into our custom peptide expression vector, expressed in rabbit reticulocyte lysate as S-labeled peptide fusions, purified on streptavidin coated agarose beads, and eluted by TEV protease cleavage of the fusion peptide.

In fact, all other vectors tested to date have proven to be unsuitable for high-level transient expression: Hairy roots infected with the TRV vector expressed at least an order of magnitude more GUS than the highest level previously reported with the replicating pBY031-I1 vector derived from Bean yellow dwarf virus.

The CRISPR/Cas9 genome editing system requires the co-expression of a Cas9 protein with a guide RNA vector expressed from the human U6 polymerase III promoter.

The results indicate that overexpression of UPF1 not only can decrease the expression of the endogenous cwp genes but also can decrease the expression of the vector-expressed cwp1 gene.

Active vaccination, passively infused neutralizing antibodies and vector-expressed CD4/CCR5 co-mimetics show promise as therapeutic immune interventions, and even more complex strategies such as HIV receptor deletion and specific destruction of integrated viral DNA sequences are progressing.

We studied whether lentiviral vector-expressed shRNA could silence GATA3 expression in differentiated human keratinocytes.

Using specifically designed shRNAs based on the E and NS5 protein genes of TMUV, the vector-expressed viral genes, TMUV RNA replication and infectious virus production were downregulated at different levels in Vero cells, where the shRNA (NS52) was highly effective in inhibiting TMUV.

We next tested whether overexpression of UPF1 influenced vector-expressed cwp1 gene.