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C.L. performed the vector design and construction.
Assuming this mechanism of CAR binding regulation is broadly applicable, it may have important implications for adenoviral vector design.
Please see the TRiP in vivo RNAi approach for information on vector design.
We have recently overcome this hurdle by rational vector design.
These results will help guide RNAi vector design.
The practicality of this vector design has been uncertain.
We propose to address this risk in future vector design.
Modlich U, Bohne J, Schmidt M, von Kalle C, Knoss S, Schambach A et al. Cell-culture assays reveal the importance of retroviral vector design for insertional genotoxicity.
Uusi-Kerttula, H., Hulin-Curtis, S., Davies, J. & Parker, A. L. Oncolytic adenovirus: strategies and insights for vector design and immuno-oncolytic applications.
This chapter covers selection of short hairpin sequences, vector design, production of lentiviral supernatants, transduction of dissociated primary hippocampal neurons, and testing the effectiveness of shRNA-mediated silencing.
Engineering of polyplexes with more dynamic domains is an encouraging new direction in nonviral vector design.
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