Sentence examples for vector cut from inspiring English sources

After cleavage with NdeI and BamHI, DNA fragments were cloned into the pET-22b vector cut with the same enzymes.

The two fragments were ligated and cloned into a pMalC2 vector cut with EcoRI and HindIII.

Subsequent digest with respective enzymes allowed sub-cloning into pUASattB vector (cut with BglII and XbaI).

The pcDNA/GL3 luciferase plasmid vector cut with the restriction enzyme Xho I was used as the control.

To study subcellular localization in vivo, Obelix-pCDNA3.1/Myc-His was cloned into the pCAβ vector (cut with BamHI and ClaI).

Ligation was done in the presence of 100 ng of annealed oligonucleotides, and 10 ng of the pLVTHM lentiviral vector cut with MluI and ClaI (a kind gift from Dr. Didier Trono, Lausanne, Switzerland).

Before excision and fixation, a vector was cut in a sagittal plane from each side of the bud complex, adjacent to the primary bud and parallel to the path of the microelectrode to aid penetration of the fixative.

The 5' end of the pUAST vector was cut with XhoI to create a sticky end.

In order to generate Col-GFP plants, here designated as Col, the open reading frame of GFP from pEZS-NLGFP vector was cut with Not I restriction enzyme and the generated DNA fragment was subsequently subcloned into the binary vector pMLBart, kindly provided by Dr. John Bowman (Monash University, Australia).

Inserts and vector were cut by AflII/ SalI and AflII/ XhoI, respectively.

The PCR product was gel purified and ligated into the Pet23a vector (Invitrogen) cut with NdeI and HindIII restriction enzymes.