Sentence examples for vector containing neomycin from inspiring English sources
A GFP-tagged Kb vector containing neomycin selected-marker was provided by Dr. Edidin [42] (Department of Biology, Johns Hopkins University, Baltimore, Maryland).
The GLO I PCR product was digested with HindIII and cloned in the vector pGEM-7zfαNeoα (containing neomycin phosphotransferase as the selection marker) to create pGEM-7zfαNeoα-LdGLOI containing the neomycin phosphotransferase gene.
The GLOI PCR product was digested with HindIII and cloned in to the vector pGEM-7zfαNeoα (containing neomycin phosphotransferase as the selection marker) with GFP at the carboxy terminal of the GLOI gene product.
These rAAV expression plasmids were subcloned into a vector containing the neomycin resistance gene.
Empty MXIE or MXIE-huTIMP-3 plasmids linearised with ScaI, and HindIII-lineared DsRed vector containing a neomycin resistance cassette, were co-transfected into the ecotropic packaging cell line GP+E-86 with Metafectene (Biontex, Germany).
A targeting vector containing a neomycin resistance gene flanked by lox P Cre-recombination sites were inserted into the intron downstream of the GASP open reading frame (ORF) (intron 5).
Renal carcinoma cell lines ACHN and 786-0 were maintained as described for HEK 293T cells and were transfected with an empty pcDNA3 vector containing a neomycin resistance gene or with the Flag-HD-PTP constructs as described above.
For stable transfection experiments, the corresponding constructs were subcloned into the complete pcDNA3 vector containing the neomycin resistance cassette.
Red fluorescent protein (RFP) was stably transfected with ds-Red (Clontech, Mountain View, CA, USA) vector containing a neomycin resistance gene in DU-145 cells.
The pFlox-PGK-Neo vector, containing a neomycin resistance gene (Neo) flanked by FRT and loxP sites and a diphtheria toxin gene cassette, was used for Ptbp2 targeting.
A mouse genomic region spanning Cox15 exons 1 2 was PCR amplified with suitable oligos to introduce a loxP site, and cloned into a vector containing a Neomycin resistance cassette, flanked by two Flp sites, and a second loxP site.
