Sentence examples for vector containing mouse from inspiring English sources
Mouse neuroblastoma cell line, N1E-115, wastablyly transfected with expression vector containing mouse NeuroD2 cDNA under HSP promoter.
In parallel, myotubes were transfected with a vector containing mouse Wnt10b cDNA.
VEGF-D-LL/2 cells were previously established by transfection with the pcDNA3.1 expression vector containing mouse VEGF-D [ 31].
The transfected murine LL/2 Lewis lung carcinoma cells with the pcDNA3.1 expression vector containing mouse VEGF-D (VEGF-D-LL/2 cells) or pcDNA3.1 (vector alone) were transfected as reported before obtained in our laboratory [ 20– 20].
The reporter vector containing mouse cytomegalovirus without the Sox2SRRS segment served as the negative control; cells transfected with this negative control vector did not show any GFP expression detectable by flow cytometry (Supplementary Figure 2).
The AAV2/9 vector containing mouse TFEB cDNA (AAV-TFEB) or GFP (AAV-GFP) driven by the CMV promoter was injected into the lateral ventricles of both cerebral hemispheres on postnatal day 0 (P0) of 5xFAD or rTg4510 mouse brains and their wild-type littermate controls.
ACAT1 overexpression was produced by transduction of cells with adenoviral vectors containing mouse ACAT1 cDNA (ACAT1-ov).
Tip60-EGFP and Hdac6-HD mutant-HA expression: ES cells were infected with pLJM1puro lentiviral vectors containing mouse Tip60 cDNA fused to EGFP.
The plasmid vector pCMV6 containing mouse cDNA of Dusp16/MKP7 was obtained from Origene (Rockville, MD, USA), and pBABE containing mouse cDNA fragment of IRS1 was from Addgene (Cambridge, MA, USA).
Following excision from the TOPO vector using Xho1 restriction enzyme, the test fragments were cloned into the pJC5-4-Xho boundassayssay vector, which is an altered form of the pGEM-4Z vector containing the mouse 5-HS2 gLCRin LCR as enhancer, a neomycin resistance gene as reporter and a chicken β-globin insulator.
Staining for HPGDS, PCNA, and CD31 was done on slides from ApcMin/+ mice with transgenic HPGDS from earlier work 5. Digoxigenin-labeled probes were prepared by in vitro transcription from a linearized plasmid vector containing the mouse PTGDR cDNA (DIG RNA labeling kit; Roche; Indianapolis, IN).
