Sentence examples for vector containing human from inspiring English sources

Yet cell-surface expression of HLA-E on porcine epithelial (LLC-PK1) cells was not observed after transfection with an expression vector harboring HLA-E alone or in combination with an expression vector containing human β2m.

Study Design: Human ovarian teratocarcinoma (PA-1) cells were transfected with an expression vector containing human zona pellucida protein 3 complementary deoxyribonucleic acid with a sequence coding for a 6-histidine tail introduced into its 3′ end.

The pLNCX vector containing human MCL-1 was a kind gift from Dr. Aly Karsan.

A pGEX expression vector containing human (isoform A) or mouse dysbindin-1 was transformed into BL21 (DE3) strain.

The Wnt4 sequence was purchased and subcloned from an Origene (Origene) vector containing human full-length Wnt4 cDNA.

Then, the gene was transferred to an expression vector containing human cytomegalovirus (CMV) promoter (pT-REx DEST30, Invitrogen) by the LR reaction using the Gateway technique.

pCDNA4TO-/Myc.his B vectors containing human full-length (WT) Beclin 1, double mutant (D121A/D124A, D146A/D149A; aspartate 'D'alanine 'A' at indicated sites), and quadruple mutant (D121A/D124A/D146A/D149A) Beclin 1 were provided by Dr. Yushan Zhu from Nankai University, Tianjin, China.

Interestingly, vectors containing human SEC61B could only be injected at very low amounts in the rescue experiments, suggesting that the Sec61β level needs to be tightly regulated.

pcDNA3.1A-MECP2A(E2 -myc and pcDNA3.1A-MECP2B(E1 -myc vectors containing human MECP2-isoform cDNAs with Myc-tag were kind gifts from Berge Minassian (Hospital for Sick Children).

pZipneo plasmid expression vectors containing human bcl-2 cDNA were used [ 21].

pMx-GFP expressing vectors containing human AID were generated by PCR-mutagenesis and co-transfected with pEco packaging vector into 293T cells using Genejuice to generate human AID-encoding retroviruses.