Given that each vector contained different promoters and cis-acting elements, their sizes were variable (Fig. 1).
Expression vectors containing different signal sequences and the gene encoding alkaline phosphatase, phoA, from Escherichia coli were constructed.
The amplified fragments without their respective signal peptide were cleaved by NcoI and XhoI and ligated into the above vectors containing different signal peptide to construct the plasmids as listed in Additional file 1: Table S1.
In this study, HIV vectors containing different combinations of cis-acting elements, including the HIV central flap sequence, and the woodchuck posttranscriptional regulatory element (WPRE) in combination with two different promoters, were used to transduce primary human lymphocytes and cord blood CD34+ progenitor cells.
The synthesized DNA fragments were inserted into the XbaI and EcoRI sites of pET-28(a) to generate vectors containing different signal peptide: pET28a-SacB, pET28a-PelB, pET28a-TorA, pET28a-WompA, pET28a-OmpASIL2, pET28a-LMSEA, pET28a-LSEAmut, pET28a-Exyl, pET28a-gIII, pET28a-STII, pET28a-XCs or pET28a-CBHI, respectively.
To define the structural domains responsible for mediating PINCH1/WT1 interaction, we generated a series of Flag-tagged, truncated PINCH1 expression vectors containing different LIM domains (Figure 6A).
MCF-7 human breast cancer cells on glass coverslides (Fisher) at 1×106 cells per 6-well tissue culture plate were transfected with vectors containing different Pak1-GFP constructs.
Analysis of TFBSs close to the integration sites provides an additional readout to study the potential genotoxicity of vectors containing different promoters, enhancers and regulatory elements in a specific cell context.
glabrata shuttle vectors containing different C. glabrata promoters and a choice of yeast-selectable markers.
The luciferase (LUC) reporter vectors containing different ERBB2 promoter fragments have been previously described (Grooteclaes et al, 1994).
