Sentence examples for vector containing cells from inspiring English sources

In both empty vector containing cells and PrPc-expressing cells, exposure of cells to CORM-2 caused a concentration-dependent reduction in the toxic effects of A β.

However, it was noted that K+ current density was significantly reduced in PrPc-expressing cells as compared with empty vector containing cells.

Neither the PrPc-expressing nor the empty vector containing cells were significantly affected by the reverse sequence peptide (A β(42-1)).

Nifedipine significantly attenuated A β toxicity in both empty vector containing cells and PrPc-expressing cells, suggesting Ca2+ influx via l-type Ca2+ channels is involved in A β toxicity.

Our study employed SH-SY5Y cells over-expressing PrPc and demonstrated that such cells were more vulnerable to the toxicity of A β when compared with the empty vector containing cells which lack endogenous PrPc expression.

All experiments were conducted using SH-SY5Y cells stably transfected with either empty pIRES neo vector (BD Biosciences, Oxford, UK; termed empty vector containing cells in this study) or engineered to express murine PrPc containing the 3F4 epitope tag (human M108/M111; termed PrPc cells), as described previously.

Whole-cell patch-clamp recordings revealed that K+ current densities, indicative of K+ channel activity at the plasma membrane, were not significantly affected by exposure to A β for 24 h in either the vector only containing cells or in the PrPc-expressing cells.

Vector containing GFP+ cells were selected by fluorescent-activated cell sorting.

Vector containing GFP+ cells were sorted by FACS (FACSAria Ilu).

To validate NPC2 protein-mediated apoptotic cell death, we measured caspase 3/7 activities from cultured H460 cells (empty vector cells and NPC2-shRNA containing cells); both cells were cultured under the identical conditions in the presence of hygromycin B. As shown in Table 1, caspase 3/7 activity in NPC2 protein-depleted cells was 13% of that of empty vector cells.

However, injection of an adenoviral vector containing glial cell line-derived neurotrophic factor significantly preserved the number of phosphor-AKT positive motor neurons and this was associated with an increase in the total number of motor neurons (Manabe et al., 2002).