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An Ad vector containing an AHA promoter-driven firefly luciferase expression cassette was prepared by an improved in vitro ligation method [ 14, 15].
MBP-IncE109 132 MBP-IncE109 132 a modified pMal-5x vector containing an additional His6 tag and tobacco etch virus-cleavable site.
Therefore, we constructed a TLMA2993 expression vector containing an artificial cDNA for TLMA2993 to generate the peptide in cells.
In this study, a pGreen 0029 vector containing an eGFP gene under the control of a Ubiquitin promoter and NptII, which is a selective marker gene, was constructed to explore and optimize the electroporation method of C. pyrenoidosa.
Proteins were produced by Escherichia coli cells transformed with the prokaryotic expression vector pET30a, namely empty vector, or the recombinant vector containing an insert of the X. oryzae hpa1 gene or its truncated version hpa1 30–126 coding for the Hpa110 42 protein.
The plasmid pMIGR1 is an MLV vector containing an IRES-GFP element [31]; pJK3 contains GagPol driven off HIV-1 LTR [32].
Human AKIP 1A, AKIP 1B, and AKIP 1C were cloned into the pEBG vector containing an amino-terminal GST epitope tag.
A modified pK7FWG2-R vector containing an additional DsRED1 marker driven by AtUBQ10 [32] was used to evaluate the transformation efficiency in different potato genotypes.
Similarly, when we intramuscularly injected a lentiviral vector containing an MSCV-EGFP cassette into murine skeletal muscles, we observed a variety of EGFP expression levels [6].
A 149bp fragment containing v311 was cloned into a luciferase pGL3 vector containing an SV40 promoter (Promega) using the Gateway cloning system (Invitrogen).
The open reading frame (ORF) of the mouse Rbm44 was cloned from testis cDNA and subcloned into pcDNA3 vector containing an N-terminal flag or myc tag sequence.
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