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The pGL3-MYB-3′-UTR vector contained a 1191 bp insert of the c-MYB 3′-UTR.
The T-DNA region of the binary vector contained a selectable marker coding for the hygromycin phosphotransferase (hpt II) gene under control of CaMV 35S promoter (provided by Dr. Su-May Yu, Academia Sinica, Taipei, Taiwan).
The pTGZC vector contained a polycistronic cassette TGZC, which was mediated by the 2A peptide.
Besides the transgene expression cassette, the T-DNA region of the binary vector contained a bar gene as a selectable marker for herbicide bialaphos resistance (Fig. 1A).
This reduction was not observed when cells were transfected with miR-147 or when the reporter vector contained a mismatch sequence (Figure 3E).
In brief, the targeting vector contained a short (0.6 kb) and a long (7.4 kb) arm of homology, a neomycin resistance cassette (neo) for positive selection, and a thymidine kinase cassette for negative selection.
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The destination vector contained an NPTII gene with the nos promoter as a selection marker.
The vector contained an N-terminal His-tag and a C-terminal RGDS sequence.
Where the binary vector contains a 1, the corresponding phase value of Parent1; where the vector contains a 0, the phase value of Parent2 is taken.
The resulting auto-associative models are excited with a preference vector containing a favorable composition of design features.
b Yeast cells were co-transformed with a bait vector, containing a promoter fragment in (a) fused to a HIS2 reporter gene, and a prey vector, containing OsWRKY80 fused to a GAL4 activation domain.
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