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Within a group of fingerprints (such as for a tissue type) the mean score of each extended pathway was then binarized (+1 if above the threshold, -1 if below) and summarized in a vector (consensus fingerprint) that represents the set of functional modules significantly over- and under-expressed in a cell type or condition.
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Therefore, a weighted consensus vector is derived from a given set of co-crystallized inhibitors with the target protein.
Theorem 1 (a.s. convergence to a finiterandom vector): Consider the consensus algorithm in (6) with initial state X ¯ ( 0 ) ∈ ℝ N M. The weight coefficients satisfy the persistence condition in (9).
This algorithm is based on three steps: (1) calculating local sufficient statistics at every node, (2) estimating a global sufficient statistics vector using a consensus filter, (3) updating parameters of the finite mixture model based on the global sufficient statistics vector.
The 630-bp product was purified and cloned into a plasmid vector and the consensus sequence determined from seven randomly selected clones; comparison of this sequence with those available in current databases for T. gondii revealed an 84.0 88.1% identity over a length of 529 bp.
Subsequently, a consensus vector is computed and then used as representative for each cluster.
A375 melanoma cells (ATCC CRL-1619) were stably transfected with a DsRed Express (Clontech) vector containing p53 consensus element [21] (p53Dsreporterreporter
This gives equal weight to the cell's current vector and the consensus orientation of its neighbours.
RACE products were cloned into a pGEM-T vector (Promega), sequenced, and consensus sequences created in Sequenchert 3.1.1 (Gene Codes Corporation).
The edited individual contigs for each species were aligned with Vector NTI to produce consensus sequences representing each species.
Moreover, using a reporter luciferase vector with NF-κB consensus sequences in its promoter, we next assessed the changes in transcriptional activity of NF-κB.
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