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DNA sequencing of the recombinant vector confirmed the accuracy of the reading frame of IL-25.
DNA sequencing of the amplicon cloned into a plasmid vector confirmed the presence of this mutation.
DNA sequencing of the recombinant vector confirmed the accuracy of the reading frame of hμPlg, and P. pastoris GS115 positive transformants were successfully identified.
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The PCA presentation of quality of storage samples (length and position of vectors) confirms the similarity of the tested samples).
Experimental data using adenovirus vectors confirm the ability of VP16 to reactivate latent genomes and that this is dependent on its acidic transactivator domain (Halford et al., 2001).
This activity band is not observed in the crude extract of the host fungus transformed with the empty vector, confirming that the observed protein is the overexpressed MpXyn10A.
Immunoblotting of protein extracts from cells given Dox with the TAK1 dominant-negative mutant K63W or the empty vector confirmed that H11/HspB8-induced Beclin-1 upregulation is TAK-1-dependent.
The overall distribution of the vector integrants confirmed the classical preferences of MLV vector to integrate inside or in the proximity of coding regions (Table 2).
Only ~25% of this level of translocation was observed for membrane vesicles prepared from cells transformed with empty vector, confirming that the bulk of the ATP-dependent movement of NBD PE was due to the presence of overexpressed MsbA.
To further examine the potential role of MECP-2 in OA pathogenesis, we modulated MECP-2 level exogenously using its specific siRNA or overexpression vector and confirmed the efficiency of MECP-2-specific siRNA or MECP overexpression vector using real-time PCR (Fig. 5B).
We introduced the amplicons into the pCR 2.1 vector (Invitrogen), confirmed the correct sequences of the insert, and sub-cloned it into a mammalian expression vector pcDNA3 (Invitrogen) with HindIII and XbaI (New England Biolabs, Ipswich, MA).
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com