Sentence examples for vector concentration of from inspiring English sources

The sediment was suspended in the buffer (25 µl) supplied with the kit and mixed with luciferase plasmid DNA/TE solution (25 µl), to yield a vector concentration of 4 µg/µl.

For comparison to the toxicity and hemolysis assays, bola-F10-G2 complexes formed at N/P = 45 with [siRNA] = 100 nM have a vector concentration of 110 μg/mL, at which no significant toxic effect was observed.

Subsequently, cells were transduced 2 hours after culturing at various vector concentrations (MOIs of 5, 10, 25 and 50) to determine the amount of lentiviral vector particles required to maximally transduce human monocyte-derived macrophages.

Amplicon stock construction and the estimation of vector concentration was described previously [14], [39], [40].

Transduction frequency was related directly to vector concentration and not to multiplicity of infection across the ranges tested.

Four parallels were made of each vector concentration.

In order to avoid dilution of the vector concentration no cell media was added to the tissue.

Required titre of virus transfected vector, drug concentration of precursor and radiation dose were lower than those of single treatment.

For the case of biochemical reaction networks the state vector x t) is the vector of concentrations of the compounds, and input signals u(t) allow to model environmental changes (as e.g. ligand concentrations, external stimuli triggering a signaling cascade, or external metabolites).

100 µl of sc-AAV5-smCBA-hGFP vector at a concentration of 4.5×1013 vector genomes/ml (produced as described by Zolotukhin, et al. [8]) was injected into the potential space.

For Bim cDNA transfection, cells were also cultured in 6-well plates until 70% confluent and transfected with control vector or BimEL expression vector, at a concentration of 4 µg in 250 µl medium, using Lipofectamine 2000.