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For that, the vector check tool was used (Medtronic, Minneapolis, MN, USA), which allows testing for the highest R-wave amplitude obtained from a single lead recording in different locations/orientations on the body surface.
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Additionally, we also used the empty pMyNT vector, to check for GroEL1 binding in the absence of a his-tagged protein.
For a received vector, codeword checking is equal to the computation of , which is (17).
That is, the pixel vector is checked to see if it lies outside the hyper ellipse whose location and shape are determined via and, respectively.
The transferred vector was checked by DNA sequencing.
The strains transformed with an integrating vector were checked by PCR with primers amplifying the corresponding promoter and reporter gene; whereas recombinant strains with replicating vectors were confirmed by recovering the plasmid after transformation into E. coli.
The resulting vector was checked by sequence analysis.
The resulting PCR products were cloned in the pCR®-BluntII-TOPO vector and checked by DNA sequencing (University of Maine DNA Sequencing Facility, Orono, ME, USA).
Each plasmid vector was checked by sequencing and includes five 10-fold serial dilutions, ranging from 10 to 10 targets per well.
A luciferase reporter construct, psi-FGFR1, was constructed by inserting a fragment of FGFR1 3′-UTR that included miR-133a/b and miR-214 binding sites into the luciferase reporter vector psi-CHECK-2.
Then they calculated motion vectors and checked if the frame had a global motion or not.
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