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Biologists also warn that the vector behind the quaggas' spread, to the Great Lakes by oceanic freighter and to the West on recreational boats, remains open to exploitation by other potentially destructive species.
Great care has been given to every actuated movement, and the results are easily the most impressive I've seen on a home robot, leaving products like Anki's Vector behind in the process.
The mtl-1 (1197bp, PstI-XbaI) and mtl-2 (551bp, XbaI/XhoI) promoter fragments were first sub-cloned into the pPD95_75 vector, and the mtl-1 and mtl-2 full length cDNA were further inserted into sites of BamHI/KpnI and XhoI/EcoRI of the pPD95_75 vector behind the fragments of Pmtl-1 and Pmtl-2, respectively, to obtain the plasmids of Pmtl-1-mtl-1 and Pmtl-2-mtl-2.
PpERG6 was inserted within the corresponding sites of the aforementioned vector behind the GFP gene.
The amplified fragment was digested with EcoR1 and Nco1 and cloned into a GFP vector behind the CaMV 35S promoter.
For overexpression, the PuORh encoding gene was cloned into the pBAD/ Myc-His A vector behind the araBAD promotor (without His-tag), and the plasmid carrying the puo gene was transformed to E. coli TOP10.
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Due to the further reduced blocking areas of porous fences, boundary-layer separation will no longer appear on the entire ground, and with the increasing porosity, path lines for velocity vectors behind the fences tend to be more horizontally stable.
The next, it is racing off, veering along some mysterious, comical vector, leaving behind a stricken golfer.
Magnetic loss is caused by the time lag of the magnetization vector M behind the magnetic field vector H.
Amplicons of the expected size were cloned into the binary vector pK7WG2 [26] behind a 35S promoter.
The HXT11 and HXT12 PCR fragments were sequenced for validation, cut using restriction enzymes XbaI and BamHI (Fermentas) and cloned in yeast expression vector pRS313-P7T7 behind the constitutive HXT7 (−391) promoter (P7) preceding the HXT7 terminator (T7).
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