Sentence examples for vector assembly from inspiring English sources

More specifically, we focus on the implementation of a "parts" based approach for suicide vector assembly, important aspects of anaerobic culturing, choices for antibiotic selection, electroporation-based DNA transformation, as well as tools for screening and verifying genetically modified constructs.

First, the implementation of global stiffness matrix assembly is described, and then, that of global force offset vector assembly is described.

PCR primers included restriction enzyme sites (underlined) compatible with the targeting vector assembly.

A first step towards achieving eventual defined composition of matter for HAC vectors [ 2] requires moving away from uncontrolled in vivo mechanisms for HAC vector assembly [ 5, 6, 8] towards construction of pre-fabricated HAC vectors containing clearly defined centromeric and other genomic elements.

The RVD ordering in the assembly vector (pFUS vector) was confirmed by sequencing.

Mori, H. et al. Expression of Bombyx mori cytoplasmic polyhedrosis virus polyhedrin in insect cells by using a baculovirus expression vector, and its assembly into polyhedra.

The target genome segment is excised from bacterial chromosomes in vitro by the RNA-guided Cas9 nuclease at two designated loci, and ligated to the cloning vector by Gibson assembly.

XB conducted vector sequence removal, assembly and analysis of EST sequences and prepared the manuscript.

Prior to sequence assembly, vector sequences were removed using SeqMan II 6.1 (DNAStar Inc., USA).

The distinction here is that the gene including its 5' and 3' regulatory sequences are rescued directly into a plant transformation vector to expedite assembly of a plant transformation construct.

When we tried a run with default settings and no quality or vector trimming, the assembly completed without any error messages but reported only 891 contigs.