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mRNA extraction was followed by cDNA preparation and the final PCR fragments were ligated into a phagemid vector as reported [49].
The T7 library was constructed with the T7Select 10-3b vector as reported in the T7Select System Manual (Novagen, Gibbstown, NJ).
A series of human APOA5 promoter fragments −846, −356, and −147 were constructed in the pGL3 luciferase vector as reported previously [ 9].
Plasmid DNA encoding either green fluorescence protein (GFP) alone, a deleted form of mouse patched lacking the second large extra cellular loop (mPtc1Δloop2) or an activated version of Gli3 (Gli3Act aa1402-4743) were inserted into pCAGGS-ires-EGFP vector as reported, for in vivo electroporation into HH11-12 stagembryosos.
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PCR products were gel-purified using the QIAquick gel extraction kit (Qiagen) and cloned onto a pCR4-TOPO® plasmid vector (Invitrogen), as reported.
The codewords are computed as c=X β T, with β belonging to the set: begin{array}{c} mathcal{B}=left(beta: beta =sqrt{frac{P}{L}}~~text{in only one of the} {M} text{elements}right. left.{vphantom{sqrt{frac{P}{L}}}}text{of each section}right) end{array} (2) Fig. 1 Construction of the sparse vector β as reported in [1].
Encoding cDNA sequence for CD9P-1, CD81 CD82, CD151 CD151 or chimeric molecules CD9×82, CD9P1-HLA-TM-Cyt, CD9P1-HLA-TM, CD9P1-HLA-Cyt were subcloned in the pCDNA3 vector (Invitrogen) as reported elsewhere [38].
Birds that attract more vectors have a higher risk for exposure to WNV, and the host's body size may be an important determinant of vector attraction, as reported for mosquitoes and sandflies (22 – 22 ).
36 The fact that 14 of the 26 study clusters were sprayed as part of the visceral leishmaniasis national control programmes during the trial had a limited impact on vector density, as reported elsewhere.
As to the introduction of RVFV in the region, some speculative hypotheses are: i) introduction through viraemic animal trading from neighbouring endemic countries or ii) wind spread of infected vectors from Mauritania, as reported in previous studies [ 1].
The pRSFDuet-1/procM-2, pRSFDuet-1/procM-2-procA3.3G 1K, pRSFDuet-1/procM-2-procA3.3G 1K, pRSFDuet-1/procM-2-procA3.3G 1KSFDuet-1/pRSFDuet-1/procM-2-procA1.7G 1R-1/procM-2-procA3.2, pRSFDuet-1/procM-2-procA1.7G 1R, pRSFDuet-1/procM-2-procA1.7G 1RG–1E pRSFDuet-1/procM-2-procA1.7G 1Red previously.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com