Sentence examples for vector as previously from inspiring English sources

The DNA sequence corresponding to a 24 amino acid region of the MPER plus a 15 amino acid linker was amplified by PCR and then cloned into the hexon HVR2 shuttle vector as previously described [16].

TBX3 was cloned into the pEF TOPO (Invitrogen, Thermo Fisher) vector as previously described11.

recombinant vector as previously described [40].

Flag tagged misfolded CPY* was generated in the pRS416Gal1 vector as previously described [31].

The oligonucleotide siRNA used for IQGAP1was cloned into pSUPER.retro.puro vector as previously described [27].

RKIP+/+ MEFs were depleted by transduction with shRNA vectors using a pLKO.1 lentiviral vector as previously described [10].

Initially, we performed viral transduction of FRG1 under the CMV promoter in the pMXIH vector as previously described [41].

Human TRF2 coding sequence was cloned into an N-terminal His-tev-Strep dual-tag affinity purification vector as previously described [56].

For the CVB4/p24 733) recombinant, the gag p24 sequence (HXB2) corresponding to amino acids 3 to 75 was amplified by PCR from a plasmid, HIV-gpt, and cloned into the CVB4 polyprotein cassette vector as previously described [16].

The influenza matrix RNA was then transcribed in vitro from the corresponding DNA template, cloned into a plasmid vector as previously described [28], and used as the standard RNA to generate standard curves for quantification of the vRNA in samples.

Lentiviral vector stocks were produced by transient transfection of 293T cells with the p8.91 encapsidation plasmid [36], the VSV glycoprotein-G-encoding pHCMV-G plasmid [37], and the lentiviral recombinant vector as previously described [38].