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This was subcloned using the pCR8/GW/TOPO TA Cloning Kit (Invitrogen, Carlsbad, CA) used in combination with Gateway compatible vectors to create an mRNA expression vector appropriate for zebrafish embryos (Villefranc et al., 2007) encoding six copies of a Myc-tag at the N-terminus of Mpi (pCS3-6×MT-Mpi).
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Briefly, the PCR amplified ORFs were recombined to generate 'entry' clones, i.e. ORFs captured in an entry or initial cloning vector that facilitates sub-cloning of the ORFs into vectors appropriate for specific experimental approaches.
The information from motion vectors is appropriate for measuring the amount of motion in a video codec, because the vectors roughly reflect the amount of motion in a block.
While this vector is not appropriate for the study of gene transcription, it is most suitable for post-transcriptional and secretion studies.
Due to the absence of pathogenicity, mild immune response, and long duration of viral genome persistence, AAV vectors appear particularly appropriate for this purpose.
The design of this vector may prove particularly appropriate for the utilization of influenza virus for the expression of heterologous proteins in their native form.
Although the vector representations are not appropriate for statistical analyses or most machine learning procedures, they are suitable for measuring distances between chemical structures, and therefore they are useful for KNN-based modeling and prediction methods.
A. tumefaciens C58C1 and a binary vector, pBIG2RHPH2, seem to be appropriate for the AMT of A. aculeatus because the transformation frequency is influenced by differences in the binary vector or bacterial strain used (Mullins et al. 2001).
It uses a vector type memory that is appropriate for growing array of objects.
We utilize a characterization of the 3-D divergence-free vector fields which is especially appropriate for a variational reformulation.
After 48 h incubation at 25 °C, the nitrocellulose discs were transferred, as appropriate for each vector, onto selection plates containing BM agar amended with 10 μg/mL sulfonylurea or YPD agar containing 200 μg/mL geneticin (G418 sodium salt) or hygromycin B and antibiotics (100 μg/mL ampicillin, 100 μg/mL cefotaxime, 100 μg/mL streptomycin and 100 μg/mL timentin).
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