Sentence examples for vector and washed from inspiring English sources

Bound antibody was detected by incubation for 30 min with ABC system (Vector), and washed 3 times in TBS, before developing with diaminobenzidine solution (DAB, Vector).

Twelve hours later, cells were transfected with 6 µg of HIV Env expression vector pNLΔGag and washed with PBS four hours' later.

Briefly, cells transfected with pcDNA3.1 or pcDNA3.1-FBLN1 vector were harvested and washed twice with PBS.

Sections were incubated with goat AP-anti-biotin tertiary (1 100, Vector) in blocking buffer and washed.

Briefly, at 24 h after transfection with vectors, SiHa cells were trypsinized and washed with cold PBS, and then resuspended in 100 μL 1× binding buffer at 1 × 106 cells/mL.

Washed and washed her hands.

Murine L1210 cells were exposed to VSV-G GFP vector particles, washed in pronase and independently propagated for up to five days, before transfer to co-culture with 293T cells.

Cells were aliquoted and transduced in separate wells with single shRNA- or control shRNA-expressing vectors, extensively washed and cultured separately for an additional 24 hr before transplantation.

After 24 h of transfection with each Tax vector or control vector, cells were washed twice with phosphate-buffered saline (PBS), fixed in 3.7% formaldehyde, permeabilized using 0.2% Triton X-100, and stained using an anti-Flag M2 MAb (Sigma), followed by an anti-mouse IgG1 Alexa 488 antibody (Molecular Probes).

SMMC-7721 and HCT116 cells trandfected witHCT116er empty vecellsor 3 x Flag-transfected expressing vector with trypsinizeithershempty PBS and fixed in ice-cold 70% ethanol in PBS.

Excessive vector was washed off by placing the muscle tissue grafts in a 50 ml Falcon tube filled with PBS.